5FOF
Crystal structure of the P.knowlesi cytosolic leucyl-tRNA synthetase editing domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-08 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 94.030, 96.820, 168.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 84.460 - 2.400 |
| R-factor | 0.22141 |
| Rwork | 0.218 |
| R-free | 0.28030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CRYPTOSPORIDIUM MURIS LEUCYL-TRNA SYNTHETASE EDITING DOMAIN |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.318 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.070 | 0.800 |
| Number of reflections | 60544 | |
| <I/σ(I)> | 15 | 2.2 |
| Completeness [%] | 99.0 | 95 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.05 M SODIUM CACODYLATE (PH 6.5), 0.2 M POTASSIUM CHLORIDE, 0.1 M MAGNESIUM ACETATE, 10% (W/V) PEG 8000, AND 25% ETHYLENEGLYCOL |
