5FOC
Crystal structure of the P.falciparum cytosolic leucyl-tRNA synthetase editing domain (space group P21)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-17 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.600, 53.490, 122.790 |
| Unit cell angles | 90.00, 97.31, 90.00 |
Refinement procedure
| Resolution | 50.010 - 1.500 |
| R-factor | 0.18543 |
| Rwork | 0.183 |
| R-free | 0.23408 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PLASMODIUM KNOWLESI LEURS EDITING DOMAIN |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.607 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.040 | 0.800 |
| Number of reflections | 110127 | |
| <I/σ(I)> | 12 | 1.5 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 3.8 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.8 | 0.2 M SODIUM NITRATE (PH 6.8), 20% (W/V) PEG 3350, AND 12% GLYCEROL |
