5FOA
Crystal Structure of Human Complement C3b in complex with DAF (CCP2-4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRUS PILATUS |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 117.429, 142.378, 323.697 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 110.390 - 4.188 |
| R-factor | 0.2714 |
| Rwork | 0.270 |
| R-free | 0.30730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2I07 1ok3 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.065 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 117.830 | 4.430 |
| High resolution limit [Å] | 4.200 | 4.200 |
| Rmerge | 0.150 | 0.590 |
| Number of reflections | 39033 | |
| <I/σ(I)> | 6.3 | 2.3 |
| Completeness [%] | 96.5 | 92.6 |
| Redundancy | 4.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 60MM MGCL2 30MM BIS-TRIS PH 5.5 6.5% PEG 3350 3% MESO-ERHYTROL |
