5FO9
Crystal Structure of Human Complement C3b in Complex with CR1 (CCP15- 17)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 1 |
| Unit cell lengths | 104.320, 113.710, 138.520 |
| Unit cell angles | 82.74, 71.77, 80.95 |
Refinement procedure
| Resolution | 60.153 - 3.300 |
| R-factor | 0.2525 |
| Rwork | 0.251 |
| R-free | 0.29130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2I07 1gkn 1gkg |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.829 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.150 | 3.480 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.160 | 0.620 |
| Number of reflections | 88061 | |
| <I/σ(I)> | 3.8 | 1.4 |
| Completeness [%] | 98.4 | 98.2 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8% PEG 3350 35MM BIS-TRIS PH 5.5 |
