5FO8

Crystal Structure of Human Complement C3b in Complex with MCP (CCP1-4)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Spacegroup name	P 21 2 21
Unit cell lengths	82.830, 130.630, 233.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.020 - 2.400
R-factor	0.1895
Rwork	0.188
R-free	0.21880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2i07 PDB ENTRY 3O8E
RMSD bond length	0.005
RMSD bond angle	0.851
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.950	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.090	0.410
Number of reflections	98854
<I/σ(I)>	6.8	2
Completeness [%]	99.0	96.2
Redundancy	3.8	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				100MM AMMONIUM CITRATE 7% PEG 3350 5MM GLUTATHIONE 50MM BIS-TRIS PROPANE, PH 6.5