5FNP
High resolution Zn containing Iron sulfur cluster repair protein YtfE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-29 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | I 41 |
| Unit cell lengths | 98.454, 98.454, 126.138 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.870 - 1.800 |
| R-factor | 0.18728 |
| Rwork | 0.186 |
| R-free | 0.21260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fnn |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.439 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.900 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.030 | 0.550 |
| Number of reflections | 51147 | |
| <I/σ(I)> | 18.11 | 2.42 |
| Completeness [%] | 92.6 | 74 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS/HCL PH 8.5 20% PEG8000 0.2 M MGCL2 |
