5FNN
Iron and Selenomethionine containing Iron sulfur cluster repair protein YtfE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-29 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | I 41 |
| Unit cell lengths | 98.620, 98.620, 125.669 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 77.580 - 2.090 |
| R-factor | 0.21201 |
| Rwork | 0.211 |
| R-free | 0.23050 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.544 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.900 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.040 | 0.440 |
| Number of reflections | 34895 | |
| <I/σ(I)> | 19.37 | 2.67 |
| Completeness [%] | 99.3 | 95.4 |
| Redundancy | 3.7 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M TRISCL PH8.5, 21% PEG4000, 0.2 M MGCL2, pH 7.5 |
