5FMD
Structure of D80A-fructofuranosidase from Xanthophyllomyces dendrorhous complexed with nystose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-16 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 74.552, 205.405, 146.362 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 119.200 - 1.780 |
| R-factor | 0.16717 |
| Rwork | 0.167 |
| R-free | 0.17782 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ann |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.371 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 119.200 | 1.810 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.070 | 0.550 |
| Number of reflections | 215107 | |
| <I/σ(I)> | 16.2 | 3.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.7 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | PROTEIN WAS CRYSTALLIZED FROM 1.25M SODIUM CITRATE TRIBASIC DIHYDRATE, THEN SOAKED IN 50MM NYSTOSE, pH 6.5 |
