5FM4
Structure of the C-terminally extended domain My4 of human myomesin (space group P21)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-25 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.530, 156.840, 48.260 |
| Unit cell angles | 90.00, 94.38, 90.00 |
Refinement procedure
| Resolution | 39.210 - 2.800 |
| R-factor | 0.2621 |
| Rwork | 0.261 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fm5 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.072 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.210 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.090 | 0.310 |
| Number of reflections | 15138 | |
| <I/σ(I)> | 10.8 | 3.1 |
| Completeness [%] | 95.3 | 89.8 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 25 % (W/V) PEG 3350 100 MM BIS-TRIS PH 5.5 |
