5FM3

Crystal structure of hyper-phosphorylated RET kinase domain with (proximal) juxtamembrane segment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 63 2 2
Unit cell lengths	98.569, 98.569, 146.301
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.665 - 2.950
R-factor	0.2004
Rwork	0.199
R-free	0.22440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NONE
RMSD bond length	0.002
RMSD bond angle	0.665
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.950
Rmerge	0.110
Number of reflections	9361
<I/σ(I)>	12.9
Completeness [%]	99.9
Redundancy	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1