5FKN
TetR(D) T103A mutant in complex with anhydrotetracycline and magnesium, P4(3)2(1)2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 67.950, 67.950, 179.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.550 - 1.800 |
| R-factor | 0.22533 |
| Rwork | 0.224 |
| R-free | 0.25542 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d7m |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.299 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.600 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 0.810 |
| Number of reflections | 39516 | |
| <I/σ(I)> | 23.7 | 2.6 |
| Completeness [%] | 98.8 | 98.2 |
| Redundancy | 12.8 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 295 | 1.4 M (NH4)2SO4, 0.1 M MES PH 6.5, 110 MM NACL, 1 MM 5A,6-ANHYDROTETRACYCLINE, 7.5 MM MGCL2 AT 295 K |
