5FKM
TetR(D) T103A mutant in complex with anhydrotetracycline and magnesium, I4(1)22
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | RIGAKU CCD |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 68.145, 68.145, 179.517 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.160 - 1.630 |
R-factor | 0.17187 |
Rwork | 0.170 |
R-free | 0.21183 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4d7m |
RMSD bond length | 0.014 |
RMSD bond angle | 1.645 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.200 | 1.730 |
High resolution limit [Å] | 1.630 | 1.630 |
Rmerge | 0.080 | 0.970 |
Number of reflections | 26692 | |
<I/σ(I)> | 21.9 | 2.4 |
Completeness [%] | 99.6 | 98.1 |
Redundancy | 10.7 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 295 | 1.2 M (NH4)2SO4, 0.1 M MES PH 6.5, 110 MM NACL, 1 MM 5A,6-ANHYDROTETRACYCLINE, 7.5 MM MGCL2 AT 295 K |