5FKC
Structure of D80A-fructofuranosidase from Xanthophyllomyces dendrorhous complexed with Raffinose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-01-23 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 74.621, 204.767, 146.502 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 119.150 - 1.820 |
R-factor | 0.17022 |
Rwork | 0.169 |
R-free | 0.18484 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ann |
RMSD bond length | 0.007 |
RMSD bond angle | 1.402 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 119.150 | 1.850 |
High resolution limit [Å] | 1.820 | 1.820 |
Rmerge | 0.070 | 0.590 |
Number of reflections | 200149 | |
<I/σ(I)> | 9.6 | 2.6 |
Completeness [%] | 99.6 | 99.5 |
Redundancy | 6.8 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | PROTEIN WAS CRYSTALLIZED FROM 1.3M SODIUM CITRATE TRIBASIC DIHYDRATE, 0.1M BIS-TRIS PROPANE PH 6.5, THEN SOAKED IN 30MM RAFFINOSE |