5FJZ
Yeast delta-COP-I mu-homology domain complexed with Dsl1 WxWxV peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-02-18 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 72.810, 72.810, 343.290 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.400 - 1.900 |
| R-factor | 0.14661 |
| Rwork | 0.145 |
| R-free | 0.18612 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fjw |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.071 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.130 | 0.710 |
| Number of reflections | 78953 | |
| <I/σ(I)> | 11.3 | 3.4 |
| Completeness [%] | 98.3 | 98.3 |
| Redundancy | 9.1 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.6 | 0.1 M HEPES PH 6.6, 2.8 M AMMONIUM SULPHATE |
