5FJW
Yeast delta-COP-I mu-homology domain complexed with Dsl1 WxWx(MSE) peptide
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-06-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97631, 0.97949, 0.97961 |
| Spacegroup name | P 1 |
| Unit cell lengths | 68.406, 79.298, 163.346 |
| Unit cell angles | 79.71, 87.50, 83.60 |
Refinement procedure
| Resolution | 160.670 - 2.800 |
| R-factor | 0.17385 |
| Rwork | 0.170 |
| R-free | 0.23949 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.683 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.600 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.150 | 1.210 |
| Number of reflections | 80910 | |
| <I/σ(I)> | 12.9 | 1.1 |
| Completeness [%] | 97.8 | 97.4 |
| Redundancy | 7 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1MHEPES-NA PH 7.0, 0.15M(NH4)2SO4, 21%W/V PEG2250 |
