5FJQ
Structural and functional analysis of a lytic polysaccharide monooxygenase important for efficient utilization of chitin in Cellvibrio japonicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.500, 50.170, 114.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.750 - 1.850 |
| R-factor | 0.22086 |
| Rwork | 0.218 |
| R-free | 0.28369 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bem |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.888 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.750 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.060 | 0.200 |
| Number of reflections | 43624 | |
| <I/σ(I)> | 13.1 | 5.4 |
| Completeness [%] | 96.9 | 96.5 |
| Redundancy | 2.25 | 2.35 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
