5FJI
Three-dimensional structures of two heavily N-glycosylated Aspergillus sp. Family GH3 beta-D-glucosidases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2009-10-17 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.519, 129.673, 217.717 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 111.410 - 1.950 |
| R-factor | 0.14983 |
| Rwork | 0.149 |
| R-free | 0.17441 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x40 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.880 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.110 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.080 | 0.440 |
| Number of reflections | 176759 | |
| <I/σ(I)> | 8.3 | 2.2 |
| Completeness [%] | 97.0 | 91.7 |
| Redundancy | 2.9 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M MIB (PACT SCREEN BUFFER)PH 5.0, 21 % PEG 1500, 25 % ETHYLENE GLYCOL |
