5FIQ
Exonuclease domain-containing 1 (Exd1) in the native conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-08 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.540, 81.580, 154.750 |
| Unit cell angles | 90.00, 111.10, 90.00 |
Refinement procedure
| Resolution | 50.010 - 2.400 |
| R-factor | 0.22226 |
| Rwork | 0.221 |
| R-free | 0.24717 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fis |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.012 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.150 | 0.970 |
| Number of reflections | 53690 | |
| <I/σ(I)> | 6.6 | 1.3 |
| Completeness [%] | 96.0 | 85.8 |
| Redundancy | 3.8 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 35% (W/V) 1,6-HEXANEDIOL, 50 MM TRIS-HCL PH 8.5, 5 MM MGSO4 |
