5FIP
Discovery and characterization of a novel thermostable and highly halotolerant GH5 cellulase from an Icelandic hot spring isolate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PIXEL |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.340, 137.450, 121.340 |
| Unit cell angles | 90.00, 114.44, 90.00 |
Refinement procedure
| Resolution | 36.380 - 1.880 |
| R-factor | 0.19519 |
| Rwork | 0.193 |
| R-free | 0.23393 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g01 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.484 |
| Data reduction software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.380 | 1.930 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.040 | 0.640 |
| Number of reflections | 126320 | |
| <I/σ(I)> | 17 | 1.8 |
| Completeness [%] | 97.5 | 90 |
| Redundancy | 3.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
