5FHT
HtrA2 protease mutant V226K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 77 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-12-17 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | H 3 |
| Unit cell lengths | 86.010, 86.010, 126.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.570 - 1.950 |
| R-factor | 0.17743 |
| Rwork | 0.175 |
| R-free | 0.22753 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | poly-alanine model of 1LCY |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.978 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.570 | |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.116 | 0.405 |
| Number of reflections | 25396 | |
| <I/σ(I)> | 7.4 | 3.1 |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 4.8 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | MES, NaCl, KH2PO4, NaH2PO4 |
