5FHK

Regulatory domain of AphB in Vibrio vulnificus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	200
Detector technology	CCD
Collection date	2015-06-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97960
Spacegroup name	P 21 21 2
Unit cell lengths	124.965, 187.926, 57.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.255 - 1.905
R-factor	0.2101
Rwork	0.208
R-free	0.25070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3szp
RMSD bond length	0.010
RMSD bond angle	1.021
Data reduction software	PHENIX
Data scaling software	HKL-2000
Refinement software	PHENIX ((1.11_2567: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.260	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.111	0.639
Number of reflections	90980
<I/σ(I)>	14.08	3.73
Completeness [%]	98.1	85.4
Redundancy	8.7	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287	0.1M HEPES:NaOH (pH 7.5), 15% PEG 8000, 10% Ethylene glycol