5FHF

Crystal structure of Bacteroides sp Pif1 in complex with ADP-AlF4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-01-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	67.090, 75.310, 111.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.780 - 2.150
R-factor	0.213
Rwork	0.211
R-free	0.25080
RMSD bond length	0.008
RMSD bond angle	0.977
Data reduction software	Aimless
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.400	2.160
High resolution limit [Å]	2.100	2.100
Number of reflections	33204
<I/σ(I)>	20.2	1.9
Completeness [%]	98.9	92.5
Redundancy	7.5	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	200 mM MgCl2, 0.1 M Tris, 10% PEG 8000