5FHE

Crystal structure of Bacteroides Pif1 bound to ssDNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-31
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	87.442, 87.442, 149.822
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.792 - 2.900
R-factor	0.2255
Rwork	0.223
R-free	0.27710
RMSD bond length	0.009
RMSD bond angle	1.154
Data reduction software	Aimless
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.800	3.080
High resolution limit [Å]	2.900	2.900
Number of reflections	15236
<I/σ(I)>	14.6	2.1
Completeness [%]	99.9	99.8
Redundancy	9.4	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1	293	4 M sodium formate, 0.1 M HEPES, 5% glycerol