5FHD

Structure of Bacteroides sp Pif1 complexed with tailed dsDNA resulting in ssDNA bound complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.99
Spacegroup name	P 21 21 21
Unit cell lengths	48.471, 149.640, 185.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.544 - 2.000
R-factor	0.1797
Rwork	0.178
R-free	0.21150
RMSD bond length	0.014
RMSD bond angle	1.224
Data reduction software	Aimless
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.030
High resolution limit [Å]	2.000	2.000
Number of reflections	91717
<I/σ(I)>	27.7	2.7
Completeness [%]	99.2	97
Redundancy	8.6	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	18% PEG 4000, 10% isopropanol, 0.1 M HEPES, 0.2 M sodium acetate