5FH9
Crystal structure of NFeoB from Escherichia coli BL21 in the apo state.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9085 |
| Spacegroup name | P 63 |
| Unit cell lengths | 166.675, 166.675, 65.581 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.115 - 3.159 |
| R-factor | 0.2088 |
| Rwork | 0.208 |
| R-free | 0.22840 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.752 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.120 | 3.272 |
| High resolution limit [Å] | 3.159 | 3.159 |
| Rmerge | 0.233 | 1.384 |
| Number of reflections | 18052 | |
| <I/σ(I)> | 9.11 | |
| Completeness [%] | 99.6 | 96.3 |
| Redundancy | 7.5 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 26.9 % PEG 8000, 0.39 M MgCl2, 0.1 M Tris-HCl pH8.0 |
