5FGS
Crystal structure of C-terminal domain of shaft pilin spaA from Lactobacillus rhamnosus GG - P21212 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | Xenocs GeniX 3D Cu HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-03-21 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 57.070, 74.343, 116.777 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.170 - 2.000 |
| R-factor | 0.21816 |
| Rwork | 0.217 |
| R-free | 0.24970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fgr |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.165 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.170 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.063 | 0.649 |
| Number of reflections | 33984 | |
| <I/σ(I)> | 24.2 | 2.9 |
| Completeness [%] | 99.3 | 97 |
| Redundancy | 9.2 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.1M MES, 0.02M zinc sulphate, 25% PEG 550MME |
