5FGP
Crystal structure of D. melanogaster Pur-alpha repeat I-II in complex with DNA.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 81.940, 40.190, 48.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.934 - 2.000 |
| R-factor | 0.1656 |
| Rwork | 0.163 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k44 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.250 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1760) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.125 | 0.793 |
| Number of reflections | 11349 | |
| <I/σ(I)> | 18.85 | 2.61 |
| Completeness [%] | 99.4 | 94.3 |
| Redundancy | 13.1 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 50 mM MES pH 5.2, 500 mM (NH4)2SO4, 1 mM TCEP and 16 % PEG400 |
