5FGC
Three dimensional structure of broadly neutralizing human anti - Hepatitis C virus (HCV) glycoprotein E2 Fab fragment HC33.8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2014-06-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00002 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 66.050, 49.570, 66.390 |
| Unit cell angles | 90.00, 98.50, 90.00 |
Refinement procedure
| Resolution | 49.570 - 1.900 |
| R-factor | 0.1962 |
| Rwork | 0.194 |
| R-free | 0.23630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.160 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 33283 | |
| <I/σ(I)> | 16.39 | 1.21 |
| Completeness [%] | 99.7 | 97.8 |
| Redundancy | 6.7 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 32% PEG 4000 100 mM Tris-HCl pH 8.5 800 mM lithium chloride Crystals obtained using heterologous microseeding. |
