5FFZ
S. aureus MepR bound to ethidium bromide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-04-27 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.54 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 119.061, 119.061, 56.279 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.015 - 2.585 |
| R-factor | 0.2479 |
| Rwork | 0.245 |
| R-free | 0.26990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eco |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.874 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.585 | 2.585 |
| Rmerge | 0.052 | 0.461 |
| Number of reflections | 4865 | |
| <I/σ(I)> | 19.32 | 3.08 |
| Completeness [%] | 99.4 | 93.93 |
| Redundancy | 8.1 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | 4M potassium formate, 0.1 M BisTris propane, pH 9, 2% PEG MME 2000 |
