5FFL
Crystal structure of mouse CD300lf at 1.6 Angstroms resolution.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-05-28 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 27.633, 53.347, 70.516 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.191 - 1.602 |
R-factor | 0.1631 |
Rwork | 0.160 |
R-free | 0.19610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xed |
RMSD bond length | 0.008 |
RMSD bond angle | 1.102 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | PHASER (2.6.0) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 1.670 |
High resolution limit [Å] | 1.600 | 3.200 | 1.600 |
Rmerge | 0.055 | 0.040 | 0.497 |
Total number of observations | 74844 | ||
Number of reflections | 13686 | ||
<I/σ(I)> | 24.8 | ||
Completeness [%] | 95.6 | 91 | 88.3 |
Redundancy | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.25 | 293 | 35% (v/v) 2-methyl-2,4-pentanediol, 50 mM sodium chloride, 120 mM Tris-HCL at pH 7.25 |