5FFJ
Structure of a nuclease-deletion mutant of the Type ISP restriction-modification enzyme LlaGI in complex with a DNA substrate mimic
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-05-20 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9797 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 87.400, 222.290, 117.410 |
Unit cell angles | 90.00, 105.14, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.840 |
R-factor | 0.231 |
Rwork | 0.229 |
R-free | 0.26210 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.767 |
Data reduction software | Aimless |
Data scaling software | SCALA |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.990 |
High resolution limit [Å] | 2.840 | 2.840 |
Rmerge | 0.590 | |
Number of reflections | 101571 | |
<I/σ(I)> | 9.6 | 2 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 291 | 100 mM Tris-HCl, 20%(w/v) PEG 20000, 4%(w/v) PEG 550 MME, 150 to 250 mM sodium acetate |