5FEY
TRIM32 RING
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.28254 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 54.536, 54.536, 99.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.781 - 2.230 |
R-factor | 0.249 |
Rwork | 0.248 |
R-free | 0.27260 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.486 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AutoSol |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.781 | |
High resolution limit [Å] | 2.230 | |
Rmerge | 0.140 | 0.962 |
Number of reflections | 7770 | |
<I/σ(I)> | 13.28 | |
Completeness [%] | 99.6 | |
Redundancy | 20 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 0.1 M MES pH 6.0 and 5 % PEG 600 |