5FEI

Crystal structure of the bacteriophage phi29 tail knob protein gp9 truncation variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	160
Detector technology	CCD
Collection date	2013-09-18
Detector	RAYONIX MX-225
Wavelength(s)	0.978
Spacegroup name	P 21 3
Unit cell lengths	184.671, 184.671, 184.671
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.355 - 2.604
R-factor	0.1682
Rwork	0.166
R-free	0.20070
Structure solution method	SIRAS
RMSD bond length	0.009
RMSD bond angle	1.179
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	130.582
High resolution limit [Å]	2.604
Number of reflections	64249
<I/σ(I)>	16.49
Completeness [%]	99.9
Redundancy	11.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.4	293.15	1.2M lithium sulfate, 10% v/v PEG 400, sodium acetate buffer, pH 4.4