5FEC
Crystal structure of 3BNC60 Fab germline precursor in complex with 426c.TM4deltaV1-3 gp120
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-06-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.033 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 103.070, 134.103, 194.955 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.424 - 3.170 |
R-factor | 0.2036 |
Rwork | 0.202 |
R-free | 0.26640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5f7e 5fa2 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.523 |
Data reduction software | XDS |
Data scaling software | Aimless (0.1.26) |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.424 | 45.850 | 3.390 |
High resolution limit [Å] | 3.170 | 8.960 | 3.170 |
Rmerge | 0.560 | 0.481 | 0.934 |
Rmeas | 0.614 | 0.529 | 1.037 |
Rpim | 0.249 | 0.215 | 0.441 |
Total number of observations | 123364 | 5589 | 17116 |
Number of reflections | 46508 | ||
<I/σ(I)> | 2.4 | 3.4 | 1.4 |
Completeness [%] | 97.7 | 99 | 87.6 |
Redundancy | 5.8 | 5.4 | 5 |
CC(1/2) | 0.730 | 0.739 | 0.350 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.1 M Imidazole pH 7.0, 8% PEG 10,000, 10 mM calcium chloride dihydrate |