5FDW
Structure of HLA-A2:01 with peptide Y10L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.73, 2.5 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.384, 79.492, 57.208 |
| Unit cell angles | 90.00, 116.09, 90.00 |
Refinement procedure
| Resolution | 48.180 - 2.700 |
| R-factor | 0.21662 |
| Rwork | 0.213 |
| R-free | 0.28505 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mre |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.457 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.190 | 0.660 |
| Number of reflections | 11732 | |
| <I/σ(I)> | 7.89 | 2.1 |
| Completeness [%] | 93.3 | 95.4 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 30% PEG 4000, 0.1M Tris-HCl pH 8.0, 0.2M lithium sulfate |
