5FDN
Crystal structure of phosphoenolpyruvate carboxylase from Arabidopsis thaliana in complex with aspartate and citrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03323 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 264.260, 268.130, 77.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.870 - 2.200 |
| R-factor | 0.1734 |
| Rwork | 0.173 |
| R-free | 0.19250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.947 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.870 | 2.500 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.239 | |
| Number of reflections | 140308 | |
| <I/σ(I)> | 19.41 | 8.48 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.4 | 7.52 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 6% PEG 6000, 100 mM sodium citrate pH 4.6 |
