5FD4
Crystal Structure of ComR from Streptococcus suis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-06-11 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.97851 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 82.271, 82.271, 209.753 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.500 - 2.900 |
| R-factor | 0.21388 |
| Rwork | 0.212 |
| R-free | 0.24208 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.096 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.500 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.081 | 1.116 |
| Number of reflections | 18768 | |
| <I/σ(I)> | 12.3 | 1.2 |
| Completeness [%] | 99.3 | 98.7 |
| Redundancy | 5.9 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2M Sodium Citrate, 15% PEG 3350 dehydration O/N with 35% PEG 8000 |
