5FCY
Structure of Anabaena (Nostoc) sp. PCC 7120 Red Carotenoid Protein binding a mixture of carotenoids
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00003 |
Spacegroup name | P 32 |
Unit cell lengths | 58.373, 58.373, 42.251 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.419 - 2.508 |
R-factor | 0.2378 |
Rwork | 0.232 |
R-free | 0.29210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xb4 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.719 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | AutoSol |
Refinement software | PHENIX ((1.10_2152: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.419 | 2.640 |
High resolution limit [Å] | 2.508 | 2.510 |
Rmerge | 0.031 | 0.156 |
Number of reflections | 5403 | |
<I/σ(I)> | 34.7 | 5.1 |
Completeness [%] | 98.0 | 83.7 |
Redundancy | 5.2 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 100 mM HEPES pH 7.5, 10% 2-propanol, 25% PEG 4000 |