5FC5

Murine SMPDL3A in complex with phosphocholine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-14
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9179
Spacegroup name	C 2 2 21
Unit cell lengths	123.734, 132.900, 80.135
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.894 - 1.678
R-factor	0.1711
Rwork	0.169
R-free	0.20360
RMSD bond length	0.013
RMSD bond angle	1.137
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	28.894
High resolution limit [Å]	1.678
Number of reflections	68190
<I/σ(I)>	17.1
Completeness [%]	90.4
Redundancy	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	0.2 M (NH4)2SO4, 0.1 M sodium acetate pH 4.6, 25% PEG 4000