5FBV
PI4KB in complex with Rab11 and the MI364 Inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.536, 102.782, 186.141 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.966 - 3.285 |
| R-factor | 0.2394 |
| Rwork | 0.237 |
| R-free | 0.28140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d0l |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.543 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.970 | 3.400 |
| High resolution limit [Å] | 3.280 | 3.280 |
| Rmerge | 0.230 | |
| Total number of observations | 50723 | |
| Number of reflections | 14745 | |
| <I/σ(I)> | 7.63 | 0.95 |
| Completeness [%] | 99.0 | |
| Redundancy | 3.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.15M amonium sulfate, 0.1M MEW pH=6, 15% PEG 4000 |
