5FB6
Room-temperature macromolecular crystallography using a micro-patterned silicon chip with minimal background scattering
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 298 |
Detector technology | PIXEL |
Collection date | 2015-03-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.003 |
Spacegroup name | I 21 3 |
Unit cell lengths | 78.820, 78.820, 78.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.867 - 1.901 |
R-factor | 0.1587 |
Rwork | 0.157 |
R-free | 0.17350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 9ins |
RMSD bond length | 0.009 |
RMSD bond angle | 0.933 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.058 | 0.372 |
Number of reflections | 6542 | |
<I/σ(I)> | 18.04 | 5.16 |
Completeness [%] | 99.2 | 99.69 |
Redundancy | 5.2 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.6 M Na2HPO4 0.01 M EDTA |