5FB2
S. aureus MepR F27L Mutant bound to oligodeoxyribonucleotide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-04-12 |
Detector | RIGAKU RAXIS HTC |
Wavelength(s) | 1.54 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 32.263, 97.986, 106.317 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.026 - 1.800 |
R-factor | 0.1921 |
Rwork | 0.188 |
R-free | 0.23180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3eco |
RMSD bond length | 0.007 |
RMSD bond angle | 0.945 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 36.030 |
High resolution limit [Å] | 1.800 |
Rmerge | 0.044 |
Number of reflections | 15799 |
<I/σ(I)> | 10.5 |
Completeness [%] | 98.1 |
Redundancy | 2.81 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | 20% (w/v) Peg 1000, 100 mM Potassium phosphate monobasic/Sodium phosphate dibasic buffer, 200 mM Sodium chloride |