5FB1

Crystal Structure of a PHD finger bound to histone H3 K9me3 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-31
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 21 21 2
Unit cell lengths	52.840, 102.964, 44.955
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.240 - 2.100
R-factor	0.1692
Rwork	0.167
R-free	0.21980
RMSD bond length	0.006
RMSD bond angle	0.817
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.10_2155: ???)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.100	5.700	2.100
Rmerge	0.116	0.038	0.665
Rmeas	0.125	0.042	0.717
Rpim	0.047	0.016	0.265
Total number of observations	105585
Number of reflections	14943
<I/σ(I)>	7.4
Completeness [%]	99.9	99.3	100
Redundancy	7.1	6.5	7.2
CC(1/2)		0.999	0.899

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	0.1 M sodium citrate/citric acid, pH 5.5, 10% 2-propanol