5FAI

EMG1 N1-Specific Pseudouridine Methyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97921
Spacegroup name	P 65 2 2
Unit cell lengths	86.118, 86.118, 125.178
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.800
R-factor	0.1684
Rwork	0.168
R-free	0.18330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v3k
RMSD bond length	0.011
RMSD bond angle	1.410
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER (2.5.7)
Refinement software	REFMAC (5.8.0131)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.093	0.031	0.912
Rmeas	0.101	0.034	0.982
Rpim	0.038	0.013	0.363
Total number of observations	181554
Number of reflections	26058
<I/σ(I)>	8.4
Completeness [%]	100.0	99.7	100
Redundancy	7	6.3	7.1
CC(1/2)		0.999	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	20% PEG 3350, 0.2 M di-NH4 Citrate