5FAA
Crystal structure of C-terminal domain of shaft pilin spaA from Lactobacillus rhamnosus GG, - I422 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97625 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 100.240, 100.240, 57.539 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.890 - 1.600 |
| R-factor | 0.1311 |
| Rwork | 0.130 |
| R-free | 0.15769 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3phs |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.221 |
| Data scaling software | Aimless |
| Phasing software | MR-Rosetta |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.890 | 1.750 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.071 | 0.604 |
| Number of reflections | 19599 | |
| <I/σ(I)> | 37 | 6.4 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 28.5 | 29.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 0.1M sodium acetate, 25% PEG400 |
