5F9Q
Crystal structure of the extracellular domain of noncanonic ABC-type transporter YknZ from Gram-positive bacteria
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2015-11-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.169, 46.147, 87.543 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.823 - 2.044 |
| R-factor | 0.1996 |
| Rwork | 0.194 |
| R-free | 0.24730 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.157 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.000 | 10.000 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.093 | |
| Number of reflections | 11741 | |
| <I/σ(I)> | 12.6 | 12.6 |
| Completeness [%] | 95.8 | 95.8 |
| Redundancy | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4.6 | 288 | 22.5% (w/v) polyethylene glycol 4000, 0.125 M ammonium acetate, 0.1 M sodium acetate trihydrate (pH 4.6) |
