5F9O

Crystal structure of broadly neutralizing VH1-46 germline-derived CD4-binding site-directed antibody CH235.09 in complex with HIV-1 clade A/E 93TH057 gp120

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-23
Detector	RAYONIX MX300-HS
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	63.530, 67.803, 225.617
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.815 - 1.860
R-factor	0.2048
Rwork	0.204
R-free	0.22050
RMSD bond length	0.016
RMSD bond angle	1.274
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.860
Number of reflections	73935
<I/σ(I)>	7.06
Completeness [%]	89.7
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	9% (w/v) PEG8000, 19% (w/v) of PEG400, 0.1 M HEPES pH 7.5