5F8J

Enterovirus 71 Polymerase Elongation Complex (C1S4 Form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97920
Spacegroup name	P 21 21 21
Unit cell lengths	63.430, 76.572, 149.267
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.425 - 2.675
R-factor	0.1851
Rwork	0.183
R-free	0.22830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ol6
RMSD bond length	0.008
RMSD bond angle	1.167
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (phenix.refine: 1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	60.000	60.000	2.760
High resolution limit [Å]	2.660	5.730	2.660
Rmerge	0.073	0.030	0.500
Rmeas	0.081	0.034	0.559
Rpim	0.035	0.015	0.241
Total number of observations	99971
Number of reflections	20430
<I/σ(I)>	9.9
Completeness [%]	96.4	92.5	98.1
Redundancy	4.9	4.8	4.9
CC(1/2)		0.999	0.845

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	0.085M MES pH 6.5, 25.5%(w/v) PEG 5000 monomethyl ether, 15%(v/v) Glycerol