5F87
Crystal structure of Drosophila Poglut1 (Rumi) complexed with UDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1000 |
| Spacegroup name | P 31 |
| Unit cell lengths | 241.908, 241.908, 47.315 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.200 |
| R-factor | 0.17016 |
| Rwork | 0.168 |
| R-free | 0.20256 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.210 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.158 | 0.349 |
| Number of reflections | 50868 | |
| <I/σ(I)> | 6.6 | 1.8 |
| Completeness [%] | 98.6 | 89.3 |
| Redundancy | 4.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M HEPES pH7.4, 1.2M sodium citrate tribasic, 6% glycerol |
