5F7G
Crystal structure of the Q108K:K40L:T51V:R58Y:Y19W:Q38L mutant of human Cellular Retinol Binding Protein II in complex with All-trans-Retinal at 1.48 Angstrom Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0781 |
| Spacegroup name | P 1 |
| Unit cell lengths | 29.970, 35.722, 64.455 |
| Unit cell angles | 90.87, 91.33, 114.36 |
Refinement procedure
| Resolution | 27.288 - 1.480 |
| R-factor | 0.2062 |
| Rwork | 0.204 |
| R-free | 0.25190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rct |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.183 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.500 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.586 | |
| Number of reflections | 43022 | |
| <I/σ(I)> | 37.3 | 2.37 |
| Completeness [%] | 95.5 | 93.6 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 25% PEG 4000, 0.1 M Ammonium Acetate, 0.1 M sodium acetate pH = 4.5 |
